DATASHEET
Host:
Rabbit
Target Protein:
NDUFS2
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
4720
Swiss Prot:
O75306
Source:
Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human NDUFS2 (NP_004541.1).
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
VALIDATION IMAGES
Lane 1: LO2 cell lysates; Lane 2: Hela cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Mouse Kidney cell lysates; Lane 6: Mouse spleen lysates; Lane 7: Rat Brain lysates probed with NDUFS2 Polyclonal Antibody, Unconjugated (bs-55147R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: NDUFS2 knockout (KO) 293T cell lysates probed with NDUFS2 Polyclonal Antibody, Unconjugated (bs-55147R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.