bs-55148R [Primary Antibody]
NDUFS3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NDUFS3

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4722

Swiss Prot: O75489

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 14kDa/30kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human liver damage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:100 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:100 overnight at 4°C, DAB staining.


Lane 1: MCF-7 cell lysates; Lane 2: SW620 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Mouse Kdiney lysates; Lane 5: Mouse Liver lysates; Lane 6: Mouse Heart lysates; Lane 7: Rat Liver lysates; Lane 8: Rat Heart lysates probed with NDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: 293T cell lysates; Lane 2: NDUFS3 knockout (KO) 293T cell lysates probed with NDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.