VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Human liver damage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with
NDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with
NDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:100 overnight at 4°C, DAB staining.
Lane 1: MCF-7 cell lysates; Lane 2: SW620 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Mouse Kdiney lysates; Lane 5: Mouse Liver lysates; Lane 6: Mouse Heart lysates; Lane 7: Rat Liver lysates; Lane 8: Rat Heart lysates probed with NDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: NDUFS3 knockout (KO) 293T cell lysates probed with NDUFS3 Polyclonal Antibody, Unconjugated (bs-55148R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.