bs-5515R
[Primary Antibody]
NFKBIA(Tyr305) Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: NFKBIA Tyr305
Modification Site: Tyr305
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4792
Swiss Prot: P25963
Source: KLH conjugated synthetic phosphopeptide derived from human IKB alpha around the phosphorylation site of Tyr305
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 35
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Yan, Xuerui, et al. "Cyanidin-3-O-glucoside Induces Apoptosis and Inhibits Migration of Tumor Necrosis Factor-α-Treated Rat Aortic Smooth Muscle Cells." Cardiovascular Toxicology (2015): 1-9.Read more>>
- Zhao, Hongyu, et al. "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway."Life Sciences (2016).Read more>>
- Li, Yuanchao, et al. "Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB." The Journal of Headache and Pain 17.1 (2016): 1-9.Read more>>
- Gao, Wenyan, et al. "Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells." Acta Histochemica (2016).Read more>>
- Tian, Yue, et al. "Minocycline attenuates sevoflurane-induced cell injury via activation of Nrf2." International Journal of Molecular Medicine.Read more>>
- Yu, Haijie, et al. "Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo." Frontiers in Pharmacology 7 (2016).Read more>>
- Liu, Xinwei, et al. "Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." Molecular Medicine Reports 16.1 (2017): 192-200.Read more>>
- Zhao et al. Fascin Overexpression Promotes Cholangiocarcinoma RBE Cell Proliferation, Migration, and Invasion. (2016) Technol.Cancer.Res.Trea. 15:322-33Read more>>
- Zhang et al. Hyperbaric Oxygen and Ginkgo Biloba Extract Ameliorate Cognitive and Memory Impairment via Nuclear Factor Kappa-B Pathway in Rat Model of Alzheimer's Disease. (2015) Chin.Med.J.(Engl. 128:3088-93Read more>>
- Chen et al. Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction. (2015) PLoS.On. 10:e0143868Read more>>
- Zhang et al. Apelin-13 protects against myocardial infarction-induced myocardial fibrosis. (2016) Mol.Med.Rep. 13:5262-8Read more>>
- Zhou J et al. Paeonol antagonizes oncogenesis of osteosarcoma by inhibiting the function of TLR4/MAPK/NF-κB pathway. Acta Histochem. 2019 Oct 3:151455. Read more>>
- Mingdong Si. et al. <em>Gentianella acuta</em> mitigates cardiovascular damage and inflammation in diet?induced hypercholesterolaemic rats. Exp Ther Med. 2021 Nov;22(5):1-1Read more>>
- Gan Rao. et al. Arsenic and polystyrene-nano plastics co-exposure induced testicular toxicity: Triggers oxidative stress and promotes apoptosis and inflammation in mice. ENVIRON TOXICOL. 2023 SepRead more>>
- Ding Lingli. et al. Ginsenoside compound-K attenuates OVX-induced osteoporosis via the suppression of RANKL-induced osteoclastogenesis and oxidative stress. NAT PRODUCT BIOPROSP. 2023 Dec;13(1):1-12Read more>>
VALIDATION IMAGES
Lane 1: mouse brain lysates; Lane 2: mouse embryo lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-10014R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 35kD. Observed band size: 27kD,35kD.
Antigen: bs-5515P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKBIA(Tyr305) Polyclonal Antibody, Unconjugated (bs-5515R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKBIA(Tyr305) Polyclonal Antibody, Unconjugated (bs-5515R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKBIA(Tyr305) Polyclonal Antibody, Unconjugated (bs-5515R) at 1:200 overnight at 4°C, DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃ and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-IKB alpha (Tyr305) Polyclonal Antibody(bs-5515R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKBIA(Tyr305) Polyclonal Antibody, Unconjugated (bs-5515R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKBIA(Tyr305) Polyclonal Antibody, Unconjugated (bs-5515R) at 1:200 overnight at 4°C, DAB staining.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (phospho-IKB alpha (Tyr305)) polyclonal Antibody, Unconjugated (bs-5515R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.