bs-55160R [Primary Antibody]
P21 Polyclonal Antibody
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Host: Rabbit

Target Protein: P21

Specificity: KO-Validated

Immunogen Range: 1-164/164


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1026

Swiss Prot: P38936

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human P21 (NP_001207707.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Size: 100µL

Concentration: 1ug/ul

Predicted Molecular Weight: 18


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang Wei. et al. C1QTNF6 regulates cell proliferation and apoptosis of NSCLC in vitro and in vivo. Bioscience Rep. 2021 Jan;41(1):BSR20201541Read more>>
  • Jiejie Yang. et al. TMEM166 inhibits cell proliferation, migration and invasion in hepatocellular carcinoma via upregulating TP53. Mol Cell Biochem. 2021 Feb;476(2):1151-1163Read more>>
  • Luya Pu. et al. Icariin arrests cell cycle progression and induces cell apoptosis through the mitochondrial pathway in human fibroblast-like synoviocytes. Eur J Pharmacol. 2021 Dec;912:174585Read more>>
  • Luya Pu. et al. TXNRD1 knockdown inhibits the proliferation of endothelial cells subjected to oscillatory shear stress via activation of the endothelial nitric oxide synthase/apoptosis pathway. BBA-MOL CELL RES. 2023 Apr;1870:119436Read more>>
  • Zixin Zhou. et al. CX3CR1hi macrophages sustain metabolic adaptation by relieving adipose-derived stem cell senescence in visceral adipose tissue. CELL REP. 2023 May;42:112424Read more>>
  • Wen Yu Zhang. et al. AMPK regulates immature boar Sertoli cell proliferation through affecting CDK4/Cyclin D3 pathway and mitochondrial function. THERIOGENOLOGY. 2024 Aug;224:9Read more>>
  • Yunping Lu. et al. Prx1/PHB2 axis mediates mitophagy in oral leukoplakia cellular senescence. PATHOL RES PRACT. 2024 Aug;260:155411Read more>>
  • Qinbing Xue. et al. Lycorine (Lycoris radiata)a unique natural medicine on breast cancer. J CELL MOL MED. 2024 Aug;28(16):e70032Read more>>
VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: P21 knockout (KO) HeLa cell lysates probed with P21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: U87-MG cell lysates probed with P21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with p21 polyclonal antibody, unconjugated (bs-55160R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.