bs-55160R-BF594

DATASHEET

Host: Rabbit

Target Protein: p21

Specificity: KO-Validated

Immunogen Range: 1-164/164

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1026

Swiss Prot: P38936

Source: Recombinant human p21

Purification: purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Conjugation: AbBy Fluor-594

Excitation/ Emission: 590nm/617nm

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)
IHC-F(1:50-200)
IHC-P(1:50-200)
IF(1:50-200)
Flow-Cyt(1:50-100)
ICC/IF(1:50-200)

Predicted Molecular Weight: 18 kDa

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.