bs-55164R [Primary Antibody]
PARP1 Polyclonal Antibody
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Host: Rabbit

Target Protein: PARP1

Specificity: KO-Validated

Immunogen Range: 81-390/1014


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 142

Swiss Prot: P09874

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Size: 100µL

Concentration: 1ug/ul

Predicted Molecular Weight: 113


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Haojie Li. et al. Calcium alleviates fluoride-induced kidney damage via FAS/FASL, TNFR/TNF, DR5/TRAIL pathways in rats. Ecotox Environ Safe. 2021 Dec;226:112851Read more>>
  • Dong-Dong Wang. et al. Identification of diterpenoids from Salvia castanea Diels f. tomentosa Stib and their antitumor activities. BIOORG CHEM. 2024 Aug;:107701Read more>>
VALIDATION IMAGES

Lane 1: Human Jurkat cell lysates; Lane 2: Human Jurkat cell lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-55164R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Hela cell lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Rat C6 cell lysates; Lane 4: Mouse testis lysates; Lane 5: Mouse brain lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-55164R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: PARP1 knockout (KO) HeLa cell lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-55164R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with PARP polyclonal antibody, unconjugated (bs-55164R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PARP1 Polyclonal Antibody, Unconjugated (bs-55164R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PARP1 Polyclonal Antibody, Unconjugated (bs-55164R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PARP1 Polyclonal Antibody, Unconjugated (bs-55164R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.