bs-55166R [Primary Antibody]
PDCD4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PDCD4

Specificity: KO-Validated

Immunogen Range: 1-260/469


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 27250

Swiss Prot: Q53EL6

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 51


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: PDCD4 knockout (KO) HeLa cell lysates probed with PDCD4 Polyclonal Antibody, Unconjugated (bs-55166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: MCF-7 cell lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Mouse Thymus lysates probed with PDCD4 Polyclonal Antibody, Unconjugated (bs-55166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.