VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with
PPP1CC Polyclonal Antibody, Unconjugated (bs-55171R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with
PPP1CC Polyclonal Antibody, Unconjugated (bs-55171R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with
PPP1CC Polyclonal Antibody, Unconjugated (bs-55171R) at 1:100 overnight at 4°C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: PPP1CC knockout (KO) HeLa cell lysates probed with PPP1CC Polyclonal Antibody, Unconjugated (bs-55171R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: HL-60 cell lysates; Lane 3: Mouse thymus lysates; Lane 4: Mouse spleen lysates; Lane 5: Rat brain lysates probed with PPP1CC Polyclonal Antibody, Unconjugated (bs-55171R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.