bs-55176R [Primary Antibody]
PRKAR2A Polyclonal Antibody
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Host: Rabbit

Target Protein: PRKAR2A

Specificity: KO-Validated

Immunogen Range: 1-404/404


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5576

Swiss Prot: P13861

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PRKAR2A (NP_004148.1).

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).

Size: 100ul, 20uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 49


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Testis lysates; Lane 3: Rat Testis lysates; Lane 4: Mouse Cerebellum lysates; Lane 5: Human K562 cell lysates; Lane 6: Human Hela cell lysates; Lane 7: Human MDA-MB-231 cell lysates; Lane 8: Human U251 cell lysates; Lane 9: Human U937 cell lysates; Lane 10: Human A673 cell lysates probed with PRKAR2A Polyclonal Antibody, Unconjugated (bs-55176R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.