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Host: Rabbit
Target Protein: RALBP1
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 10928
Swiss Prot: Q15311
Source: Recombinant fusion protein containing a sequence corresponding to amino acids 396-655 of human RALBP1 (NP_006779.1).
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Size: 100µL
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IHC
Predicted Molecular Weight: 76kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Hela cell lysates; Lane 2: RALBP1 knockout (KO) HeLa cell lysates probed with RALBP1 Polyclonal Antibody, Unconjugated (bs-55186R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human A673 cell lysates; Lane 2: Mouse spleen lysates; Lane 3: Mouse lung lysates probed with RALBP1 Polyclonal Antibody, Unconjugated (bs-55186R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RALBP1 Polyclonal Antibody, Unconjugated (bs-55186R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RALBP1 Polyclonal Antibody, Unconjugated (bs-55186R) at 1:100 overnight at 4°C, DAB staining.