bs-55189R [Primary Antibody]
S100A11 Polyclonal Antibody
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Host: Rabbit

Target Protein: S100A11

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6282

Swiss Prot: P31949

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human S100A11 (NP_005611.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ICC/IF(1:50-200)

Predicted Molecular Weight: 11kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: BxPC-3 cell lysates; Lane 2: BT-474 cell lysates; Lane 3: Lovo cell lysates; Lane 4: 22Rv1 cell lysates; Lane 5: SKOV3 cell lysates; Lane 6: THP-1 cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: S100A11 knockout (KO) HeLa cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human HeLa cell lysates; Lane 2: Human U-2 OS cell lysates; Lane 3: Human DU145 cell lysates; Lane 4: Human MCF-7 cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:400 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.