VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:100 overnight at 4°C, DAB staining.
Lane 1: BxPC-3 cell lysates; Lane 2: BT-474 cell lysates; Lane 3: Lovo cell lysates; Lane 4: 22Rv1 cell lysates; Lane 5: SKOV3 cell lysates; Lane 6: THP-1 cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: S100A11 knockout (KO) HeLa cell lysates probed with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (S100A11) polyclonal Antibody, Unconjugated (bs-55189R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.