DATASHEET
Host:
Rabbit
Target Protein:
S100A6
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
6277
Swiss Prot:
P06703
Source:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6 (NP_055439.1).
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.
VALIDATION IMAGES
Lane 1: Hela cell lysates; Lane 2: S100A6 knockout (KO) HeLa cell lysates probed with S100A6 Polyclonal Antibody, Unconjugated (bs-55190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: NIH/3T3 cell lysates; Lane 3: HT-29 cell lysates; Lane 4: Mouse Lung lysates; Lane 5: Mouse skeletal muscle lysates probed with S100A6 Polyclonal Antibody, Unconjugated (bs-55190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.