bs-55193R [Primary Antibody]
SCD Polyclonal Antibody
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Host: Rabbit

Target Protein: SCD

Specificity: KO-Validated

Immunogen Range: 200-359/359


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6319

Swiss Prot: O00767

Source: A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human SCD (NP_005054.3).

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17.

Size: 100ul, 20uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-F(1:100-500)
IF(ICC)(1:50-200)
IF(1:50-200)
IHC-P(1:200-400)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with SCD polyclonal antibody, unconjugated (bs-55193R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SCD Polyclonal Antibody, Unconjugated (bs-55193R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.