bs-55194R [Primary Antibody]
SEC61B Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SEC61B

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10952

Swiss Prot: P60468

Source: A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 9kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SEC61B) polyclonal Antibody, Unconjugated (bs-55194R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Lane 1: 293T cell lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: SH-SY5Y cell lysates; Lane 5: Mouse Brain lysates; Lane 6: Mouse Stomach lysates probed with SEC61B Polyclonal Antibody, Unconjugated (bs-55194R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: 293T cell lysates; Lane 2: SEC61B knockout (KO) 293T cell lysates probed with SEC61B Polyclonal Antibody, Unconjugated (bs-55194R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.