bs-55197R [Primary Antibody]
SGTA Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SGTA

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6449

Swiss Prot: O43765

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SGTA (NP_003012.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 34kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: A549 cell lysates; Lane 3: U-251MG cell lysates probed with SGTA Polyclonal Antibody, Unconjugated (bs-55197R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: 293T cell lysates; Lane 2: SGTA knockout (KO) 293T cell lysates probed with SGTA Polyclonal Antibody, Unconjugated (bs-55197R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SGTA) polyclonal Antibody, Unconjugated (bs-55197R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.