bs-55204R [Primary Antibody]
SMARCC1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SMARCC1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6599

Swiss Prot: Q92922

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1 (NP_003065.3).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 122kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human kidney cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SMARCC1 Polyclonal Antibody, Unconjugated (bs-55204R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Hela cell lysates; Lane 2: SMARCC1 knockout (KO) HeLa cell lysates probed with SMARCC1 Polyclonal Antibody, Unconjugated (bs-55204R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: BT-474 cell lysates; Lane 2: SW620 cell lysates; Lane 3: 293T cell lysates probed with SMARCC1 Polyclonal Antibody, Unconjugated (bs-55204R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.