Host: Rabbit
Target Protein: TFAP4
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7023
Swiss Prot: Q01664
Source: Synthetic Peptide of human TFAP4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 38kDa
Cross Reactive Species:
Human
Rat
For research use only. Not intended for diagnostic or therapeutic use.
C6 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TFAP4) polyclonal Antibody, Unconjugated (bs-55214R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TFAP4) polyclonal Antibody, Unconjugated (bs-55214R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Hela cell lysates; Lane 2: TFAP4 knockout (KO) HeLa cell lysates probed with TFAP4 Polyclonal Antibody, Unconjugated (bs-55214R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HT-29 cell lysates; Lane 2: SGC7901 cell lysates; Lane 3: 22Rv1 cell lysates probed with TFAP4 Polyclonal Antibody, Unconjugated (bs-55214R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.