bs-55216R [Primary Antibody]
TMPO Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TMPO

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7112

Swiss Prot: P42166/P42167

Source: Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 38kDa/50kDa/75kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TMPO Polyclonal Antibody, Unconjugated (bs-55216R) at 1:100 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TMPO Polyclonal Antibody, Unconjugated (bs-55216R) at 1:100 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TMPO Polyclonal Antibody, Unconjugated (bs-55216R) at 1:100 overnight at 4°C, DAB staining.


Lane 1: MCF-7 cell lysates; Lane 2: 293T cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Mouse Liver lysates; Lane 5: Rat Liver lysates probed with TMPO Polyclonal Antibody, Unconjugated (bs-55216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: 293T cell lysates; Lane 2: TMPO knockout (KO) 293T cell lysates probed with TMPO Polyclonal Antibody, Unconjugated (bs-55216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.