bs-5577R [Primary Antibody]
phospho-PTPN6 (Tyr536) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: phospho-PTPN6 (Tyr536)

Modification Site: Tyr536

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5777

Swiss Prot: P29350

Source: KLH conjugated synthetic phosphopeptide derived from human PTPN6 around the phosphorylation site of Tyr536 [SE(p-Y)GN]

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

SHP1 is a member of the non receptor protein tyrosine phosphatase subfamily. The PTP family comprises of at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is cytosolic. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. SHP1 is implicated in the control of tyrosine kinase signalling pathways in cellular proliferation, with a potential role in cancer.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 65


Cross Reactive Species: Human

Predicted Cross Reactive Species: Dog
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lopes, Filipa B., et al. "Membrane nanoclusters of FcγRI segregate from inhibitory SIRPα upon activation of human macrophages." J Cell Biol (2017): jcb-201608094.Read more>>
  • Mkaddem et al. Lyn and Fyn function as molecular switches that control immunoreceptors to direct homeostasis or inflammation. (2017) Nat.Commun. 8:246Read more>>
  • Mkaddem et al. Lyn and Fyn function as molecular switches that control immunoreceptors to direct homeostasis or inflammation. (2017) Nat.Commun. 8:246Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with PTPN6(Tyr536) Polyclonal Antibody (bs-5577R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.