bs-5591R [Primary Antibody]
phospho-YES1 (Tyr426) Polyclonal Antibody
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Host: Rabbit

Target Protein: phospho-YES1 (Tyr426)

Specificity: Due to the highly conserved nature of this protein, this antibody will react with the related proteins LCK(Tyr394), Src(Tyr418), and Lyn (Tyr397) if phosphorylated at this site due to homology.

Immunogen Range: 420-430/543


Modification Site: Tyr426

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7525

Swiss Prot: P07947

Source: KLH conjugated synthetic phosphopeptide derived from human YES1 around the phosphorylation site of Tyr426

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 61


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Chicken
Rabbit
GuineaPig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-phospho-YES1(Tyr426) Polyclonal Antibody, Unconjugated (bs-5591R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Rat Embryo tissue lysates probed with phospho-YES1 (Tyr426) Polyclonal Antibody, Unconjugated (bs-5591R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.