bs-5758R
[Primary Antibody]
FAP Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: FAP
Immunogen Range: 311-410/760
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2191
Swiss Prot: Q12884
Source: KLH conjugated synthetic peptide derived from human FAPA
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 88
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhang, Bo, et al. "Enhanced anti-tumor activity of EGFP-EGF1-conjugated nanoparticles by a multi-targeting strategy." ACS Applied Materials & Interfaces (2016).Read more>>
- Miller, Anne, et al. "Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments." Cell Metabolism(2017).Read more>>
- Chen et al. Stromal fibroblasts derived from mammary gland of bovine with mastitis display inflammation-specific changes. (2016) Sci.Rep. 6:27462Read more>>
- Junyoung Kim. et al. Three-Dimensional Human Liver-Chip Emulating Premetastatic Niche Formation by Breast Cancer-Derived Extracellular Vesicles. Acs Nano. 2020;14(11):14971C14988Read more>>
- Li-Fang Shen. et al. The role of Interleukin 36 in the Epithelial-Mesenchymal Transition process of Chronic Rhinosinusitis: a pilot study. CLIN OTOLARYNGOL. 2022 OcRead more>>
- Hocheol Shin. et al. Nanovaccine Displaying Immunodominant T Cell Epitopes of Fibroblast Activation Protein Is Effective Against Desmoplastic Tumors. ACS NANO. 2023;XXXX(XXX):XXX-XXXRead more>>
- Chao Chen. et al. Downregulation of miR-100-5p in cancer-associated fibroblast-derived exosomes facilitates lymphangiogenesis in esophageal squamous cell carcinoma. CANCER MED-US. 2023 MayRead more>>
- Miaomiao Sun. et al. Cancer-associated fibroblast-derived exosome microRNA-21 promotes angiogenesis in multiple myeloma. SCI REP-UK. 2023 Jun;13(1):1-13Read more>>
- Nataliya Basalova. et al. Mesenchymal stromal cells facilitate resolution of pulmonary fibrosis by miR-29c and miR-129 intercellular transfer. EXP MOL MED. 2023 Jul;:1-14Read more>>
- Zhiping Wang. et al. Prognostic and immunological role of cancer-associated fibroblasts-derived exosomal protein in esophageal squamous cell carcinoma. INT IMMUNOPHARMACOL. 2023 Nov;124:110837Read more>>
- Zhenfeng Liu. et al. Fibroblast Activation ProteinTargeted PET/CT with Al18F-NODA-FAPI-04 for In Vivo Imaging of Tendon Healing in Rat Achilles Tendon Injury Models. AM J SPORT MED. ;()Read more>>
- Chenglin Liang. et al. Spatiotemporal transformable nano-assembly for on-demand drug delivery to enhance anti-tumor immunotherapy. ASIAN J PHARM SCI. 2024 Feb;:100888Read more>>
- Liu Zao-Ling. et al. WGCNA reveals a biomarker for cancer-associated fibroblasts to predict prognosis in cervical cancer. J CHIN MED ASSOC. 2024 Jul;:10.1097/JCMA.0000000000001129Read more>>
VALIDATION IMAGES
Lane 1: Human U87MG cell lysates; Lane 2: Human Siha cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human A549 cell lysates probed with FAPA Polyclonal Antibody, Unconjugated (bs-5758R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Polyclonal Antibody, Unconjugated (bs-5758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Polyclonal Antibody, Unconjugated (bs-5758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Polyclonal Antibody, Unconjugated (bs-5758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Polyclonal Antibody, Unconjugated (bs-5758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Polyclonal Antibody, Unconjugated (bs-5758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Polyclonal Antibody, Unconjugated (bs-5758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Polyclonal Antibody, Unconjugated (bs-5758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Polyclonal Antibody, Unconjugated (bs-5758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.