bs-5758R [Primary Antibody]
FAPA Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FAPA

Immunogen Range: 311-410/760


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2191

Swiss Prot: Q12884

Source: KLH conjugated synthetic peptide derived from human FAPA

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 88


Cross Reactive Species: Human
Mouse
Bovine

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Bo, et al. "Enhanced anti-tumor activity of EGFP-EGF1-conjugated nanoparticles by a multi-targeting strategy." ACS Applied Materials & Interfaces (2016).Read more>>
  • Miller, Anne, et al. "Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments." Cell Metabolism(2017).Read more>>
  • Chen et al. Stromal fibroblasts derived from mammary gland of bovine with mastitis display inflammation-specific changes. (2016) Sci.Rep. 6:27462Read more>>
  • Junyoung Kim. et al. Three-Dimensional Human Liver-Chip Emulating Premetastatic Niche Formation by Breast Cancer-Derived Extracellular Vesicles. Acs Nano. 2020;14(11):14971C14988Read more>>
  • Li-Fang Shen. et al. The role of Interleukin 36 in the Epithelial-Mesenchymal Transition process of Chronic Rhinosinusitis: a pilot study. CLIN OTOLARYNGOL. 2022 OcRead more>>
  • Hocheol Shin. et al. Nanovaccine Displaying Immunodominant T Cell Epitopes of Fibroblast Activation Protein Is Effective Against Desmoplastic Tumors. ACS NANO. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Chao Chen. et al. Downregulation of miR-100-5p in cancer-associated fibroblast-derived exosomes facilitates lymphangiogenesis in esophageal squamous cell carcinoma. CANCER MED-US. 2023 MayRead more>>
  • Miaomiao Sun. et al. Cancer-associated fibroblast-derived exosome microRNA-21 promotes angiogenesis in multiple myeloma. SCI REP-UK. 2023 Jun;13(1):1-13Read more>>
  • Nataliya Basalova. et al. Mesenchymal stromal cells facilitate resolution of pulmonary fibrosis by miR-29c and miR-129 intercellular transfer. EXP MOL MED. 2023 Jul;:1-14Read more>>
  • Zhiping Wang. et al. Prognostic and immunological role of cancer-associated fibroblasts-derived exosomal protein in esophageal squamous cell carcinoma. INT IMMUNOPHARMACOL. 2023 Nov;124:110837Read more>>
  • Zhenfeng Liu. et al. Fibroblast Activation ProteinTargeted PET/CT with Al18F-NODA-FAPI-04 for In Vivo Imaging of Tendon Healing in Rat Achilles Tendon Injury Models. AM J SPORT MED. ;()Read more>>
  • Chenglin Liang. et al. Spatiotemporal transformable nano-assembly for on-demand drug delivery to enhance anti-tumor immunotherapy. ASIAN J PHARM SCI. 2024 Feb;:100888Read more>>
VALIDATION IMAGES

Lane 1: Human U87MG cell lysates; Lane 2: Human Siha cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human A549 cell lysates probed with FAPA Polyclonal Antibody, Unconjugated (bs-5758R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.