Host: Rabbit
Target Protein: FAP1
Immunogen Range: 801-900/2485
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5783
Swiss Prot: Q12923
Source: KLH conjugated synthetic peptide derived from human FAP1/PTPN13
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Conjugation: ALEXA FLUOR® 647
Excitation/ Emission: 650nm/665nm
Size: 100ul
Concentration: 1ug/ul
Applications:
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 273
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Chia-Hao Huang. et al. Cell marathon: long-distance cell migration and metastasis-associated gene analysis using a folding paper system. LAB CHIP. 2022 SepRead more>>
HL-60 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FAP1 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-5760R-AF647) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
A431 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FAP1 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-5760R-AF647) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).