bs-5794R [Primary Antibody]
Robo1/2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Robo1/2

Immunogen Range: 51-150/1651


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6091, 6092

Source: KLH conjugated synthetic peptide derived from human Robo1/2.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior (1). In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively (1). These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway (1). One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals (2,3). Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion (1-3). Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross (1). Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout (2-4). Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray (5,6).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 181


Cross Reactive Species: Rat
Chicken

Predicted Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016).Read more>>
VALIDATION IMAGES

Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; \nAntigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Blocking buffer (normal goat serum 5%) at 37℃ for 20 min; \nIncubation: Anti-Robo Polyclonal Antibody, Unconjugated (bs-5794R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.