bs-5810R [Primary Antibody]
Lactoferrin Polyclonal Antibody
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Host: Rabbit

Target Protein: Lactoferrin

Immunogen Range: 611-710/710


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4057

Source: KLH conjugated synthetic peptide derived from human Lactoferrin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 76


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao et al. Neutrophil polarization by IL-27 as a therapeutic target for intracerebral hemorrhage. (2017) Nat.Commun. 8:602Read more>>
  • Carman Man et al. Aging-Associated Alterations in Mammary Epithelia and Stroma Revealed by Single-Cell RNA SequencingCell Rep.2020 Dec 29;33(13):108566.Read more>>
  • Tsatsanis, Andrew. et al. The acute phase protein lactoferrin is a key feature of Alzheimers disease and predictor of A burden through induction of APP amyloidogenic processing. Mol Psychiatr. 2021 Aug;:1-16Read more>>
  • Zhongnan Xiao. et al. Reduction of lactoferrin aggravates neuronal ferroptosis after intracerebral hemorrhagic stroke in hyperglycemic mice. Redox Biol. 2022 Apr;50:102256Read more>>
VALIDATION IMAGES

Human breast milk probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n


Human Lactoferrin protein (100ng) lysates probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Polyclonal Antibody(bs-5810R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


25 ug total protein per lane of various lysates (see on figure) probed with Lactoferrin polyclonal antibody, unconjugated (bs-5810R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.