bs-5812R [Primary Antibody]
PHLP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PHLP

Immunogen Range: 51-150/301


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5082

Swiss Prot: Q13371

Source: KLH conjugated synthetic peptide derived from human PHLP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Isoform 1: Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers. Isoform 2: Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 34


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Rat Heart tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Rat Kidney tissue lysates; Lane 7: Human HUVEC cell lysates; Lane 8: Human K562 cell lysates probed with PHLP Polyclonal Antibody, Unconjugated (bs-5812R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.