Host: Rabbit
Target Protein: ADAM23
Immunogen Range: 551-650/832
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 8745
Swiss Prot: O75077
Source: KLH conjugated synthetic peptide derived from human ADAM23
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 75-95
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Pingping Yao. et al. Identification of ADAM23 as a Potential Signature for Psoriasis Using Integrative Machine-Learning and Experimental Verification. INT J GEN MED. 2023 Dec 31Read more>>
Raji lysate probed with Rabbit Anti-ADAM23 Polyclonal Antibody (bs-5853R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ADAM23 Polyclonal Antibody, Unconjugated (bs-5853R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with ADAM23 Polyclonal Antibody, Unconjugated (bs-5853R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.