Host: Rabbit
Target Protein: Endomucin
Immunogen Range: 181-261/261
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 51705
Swiss Prot: Q9ULC0
Source: KLH conjugated synthetic peptide derived from human Endomucin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 25
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
For research use only. Not intended for diagnostic or therapeutic use.
- Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12Read more>>
- Jie Mi. et al. Implantable Electrical Stimulation at Dorsal Root Ganglions Accelerates Osteoporotic Fracture Healing via Calcitonin Gene-Related Peptide. 2021 Oct 28Read more>>
- Weizhen Chen. et al. An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties. Biomaterials. 2021 Nov;:12126Read more>>
- Xu, Xi. et al. PD-1 signalling defines and protects leukaemic stem cells from T?cell?receptor-induced cell death in T?cell acute lymphoblastic leukaemia. NAT CELL BIOL. 2023 Jan;:1-13Read more>>
- Jinxiu Yu. et al. Promoting osseointegration of titanium by pH-responsive releasing of H2S and optimizing polarization time for macrophages. COMPOS PART B-ENG. 2023 Mar;253:110554Read more>>
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Endomucin Polyclonal Antibody, Unconjugated (bs-5884R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Endomucin) polyclonal Antibody, Unconjugated (bs-5884R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.