bs-5888R [Primary Antibody]
Hyaluronidase2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Hyaluronidase2

Immunogen Range: 111-210/473


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8692

Swiss Prot: Q12891

Source: KLH conjugated synthetic peptide derived from human HYAL2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 49


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Petra _dnkov. et al. The Degradation of Hyaluronan in the Skin. Biomolecules. 2022 Feb;12(2):251Read more>>
  • Anna Kocurkova. et al. Endogenously produced hyaluronan contributes to the regulation of peritoneal adhesion development. BIOFACTORS. 2023 MayRead more>>
  • Romana ?nov. et al. The hyaluronan metabolism in the UV-irradiated human epidermis and the relevance of in vitro epidermal models. EXP DERMATOL. 2023 JulRead more>>
VALIDATION IMAGES

Mouse testis lysate probed with Rabbit Anti-Hyaluronidase 2 Polyclonal Antibody (bs-5888R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-Hyaluronidase2 Polyclonal Antibody, Unconjugated (bs-5888R) at 1:200 followed by conjugation to the secondary antibody and DAB staining