Host: Rabbit
Target Protein: SET
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6418
Swiss Prot: Q01105
Source: KLH conjugated synthetic peptide derived from human SET
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
FCM(FCM=1:100)
ICC(ICC=1:25)
Predicted Molecular Weight: 32
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Yabe et al. Characterization of SET/I2PP2A isoforms in dogs. (2014) J.Vet.Med.Sc. 76:1235-40Read more>>
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SET) polyclonal Antibody, Unconjugated (bs-5943R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human HepG2 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human MOLT4 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A431 cell lysates probed with SET Polyclonal Antibody, Unconjugated (bs-5943R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.