bs-5968R-FITC [Conjugated Primary Antibody]
NARG2 Polyclonal Antibody, FITC Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: NARG2

Immunogen Range: 201-300/982


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 79664

Swiss Prot: Q659A1

Source: KLH conjugated synthetic peptide derived from human NARG2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 110


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Schmidt et al. Regulation of the oncogenic phenotype by the nuclear body protein ZC3H8. (2018) BMC.Cancer. 18:759Read more>>
VALIDATION IMAGES

U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NARG2 Polyclonal Antibody(bs-5968R-FITC)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).