bs-5973R-A680 [Conjugated Primary Antibody]
SIRT7 Polyclonal Antibody, ALEXA FLUOR® 680 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SIRT7

Immunogen Range: 231-330/400


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 51547

Swiss Prot: Q9NRC8

Source: KLH conjugated synthetic peptide derived from human SIRT7

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.

Conjugation: ALEXA FLUOR® 680

Excitation/ Emission: 679nm/702nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 45


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Takumida, Masaya, Hiroshi Takumida, and Matti Anniko. "Localization of sirtuins in the mouse inner ear." Acta Oto-Laryngologica 0 (2014): 1-8.Read more>>
  • Wronska, A., et al. "Age-Related Changes in Sirtuin 7 Expression in Calorie-Restricted and Refed Rats." Gerontology (2015).Read more>>
  • Deng Disi. et al. Protective Effect of XinJiaCongRongTuSiZiWan on the Reproductive Toxicity of Female Rats Induced by Triptolide. EVID-BASED COMPL ALT. 2022;2022:3642349Read more>>
VALIDATION IMAGES