bs-5983R [Primary Antibody]
AARE Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AARE

Immunogen Range: 101-200/732


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 327

Source: KLH conjugated synthetic peptide derived from human AARE

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

AARE (Acylamino-acid-releasing enzyme) is also known as Acyl-peptide hydrolase. It catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus corresponding to the protein are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 81


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney labeled with Anti-AARE Polyclonal Antibody, Unconjugated (bs-5983R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: Hela cell lysates;Lane 2: Hl60 cell lysates;Lane 3: U2OS cell lysates; Lane 4: U87mg cell lysates probed with AARE Polyclonal Antibody, Unconjugated (bs-5983R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Kidney tissue lysates; Lane 2: Human U-2 OS cell lysates probed with AARE Polyclonal Antibody, Unconjugated (bs-5983R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.