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Host: Rabbit
Target Protein: ABI3
Immunogen Range: 151-250/366
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 51225
Swiss Prot: Q9P2A4
Source: KLH conjugated synthetic peptide derived from human ABI3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
Predicted Molecular Weight: 39
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-ABI3 Polyclonal Antibody, Unconjugated (bs-5985R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ABI3 Polyclonal Antibody, Unconjugated(bs-5985R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
25 ug total protein per lane of various lysates (see on figure) probed with ABI3 polyclonal antibody, unconjugated (bs-5985R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with ABI3 polyclonal antibody, unconjugated (bs-5985R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.