bs-60036R [Primary Antibody]
Acetyl-Histone H4 (Lys16) Polyclonal Antibody
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Host: Rabbit

Target Protein: Acetyl-Histone H4 (Lys16)

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P62805

Purification: Protein A coupled with the antigen peptide containing acetylated H4 (Lys16).

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: Lot Dependent

Applications: WB
IHC-F
IF
IHC-P
ICC/IF

Cross Reactive Species: Human
Mouse
Rat
Rice

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H4 (Lys16) Polyclonal Antibody, Unconjugated (bs-60036R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Histone H4 (acetyl K16) polyclonal antibody, unconjugated (bs-60036R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.