bs-60071R [Primary Antibody]
phospho-Histone H3 (Ser10) Polyclonal Antibody
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Host: Rabbit

Target Protein: phospho-Histone H3 (Ser10)

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8290

Swiss Prot: Q16695

Source: KLH conjugated synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:500-2000)
IHC-F(1:50-200)
IF(1:50-200)
IHC-P(1:500-2000)

Predicted Molecular Weight: 15


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

NIH/3T3 (M)+Serum (M)(0%, overnight) add Nocodazole(100ng/mL, 18h) add Calyculin A (100nM, 1h), C6 (R) treated with calyculin A(100nM) for 30 min, HeLa (H) treated with calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3 (Ser10) polyclonal antibody, unconjugated (bs-60071R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Polyclonal Antibody, Unconjugated (bs-60071R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Polyclonal Antibody, Unconjugated (bs-60071R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Polyclonal Antibody, Unconjugated (bs-60071R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.