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Host: Rabbit
Target Protein: Mono-Methyl-Histone H3 (Lys4)
Clonality: Polyclonal
Isotype: IgG
Swiss Prot: P68431
Purification: Protein A coupled with the antigen peptide containing mono-methylated H3 (Lys4).
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µl
Concentration: Lot Dependent
Applications:
WB
IHC-F
IF
IHC-P
ICC/IF
Predicted Molecular Weight: 17
Cross Reactive Species:
Human
Mouse
Rat
Monkey
For research use only. Not intended for diagnostic or therapeutic use.
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: HeLa, C2C12, COS-7 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 17 kDa Observed MW: 17 kDa
Tissue: Mouse colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bs-60097R
25 ug total protein per lane of various lysates (see on figure) probed with Mono-Methyl-Histone H3 (Lys4) polyclonal antibody, unconjugated (bs-60097R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.