bs-60163R [Primary Antibody]
Mono-Methyl-Histone H4 (Arg19) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Mono-Methyl-Histone H4 (Arg19)

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P62805

Purification: Protein A coupled with the antigen peptide containing H4R19me1.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:300-5000)

Predicted Molecular Weight: 11


Cross Reactive Species: Human
Mouse
Rat
Rice

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: HeLa, H1299, 293T, C2C12 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 11 kDa Observed MW: 11 kDa


Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Mouse liver, Mouse spleen Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 11 kDa Observed MW: 11 kDa


Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Rice, N2a, Mouse spleen Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 11 kDa Observed MW: 11 kDa