bs-6020R [Primary Antibody]
ACVRL1 Polyclonal Antibody
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Host: Rabbit

Target Protein: ACVRL1

Immunogen Range: 30-80/503


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 94

Swiss Prot: P37023

Source: KLH conjugated synthetic peptide derived from human ALK1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 56


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti ACVRL1/ALK1 Polyclonal Antibody, Unconjugated (bs-6020R) at 1:100 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse cancer tissue labeled with Anti ACVRL1/ALK1 Polyclonal Antibody, Unconjugated (bs-6020R) at 1:300 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti ACVRL1/ALK1 Polyclonal Antibody, Unconjugated (bs-6020R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti ACVRL1/ALK1 Polyclonal Antibody, Unconjugated (bs-6020R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 53kD. Observed band size: 53kD.


Paraformaldehyde-fixed, paraffin embedded (rat placenta）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ACVRL1 ) Polyclonal Antibody, Unconjugated (bs-6020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat liver）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ACVRL1 ) Polyclonal Antibody, Unconjugated (bs-6020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse liver）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ACVRL1 ) Polyclonal Antibody, Unconjugated (bs-6020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human placenta）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ACVRL1 ) Polyclonal Antibody, Unconjugated (bs-6020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human lung）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ACVRL1 ) Polyclonal Antibody, Unconjugated (bs-6020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ACVRL1 ) Polyclonal Antibody, Unconjugated (bs-6020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ACVRL1 ) Polyclonal Antibody, Unconjugated (bs-6020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.