bs-6028R [Primary Antibody]
CD16 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD16a/CD16b

Immunogen Range: 145-195/254

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2214

Swiss Prot: P08637

Source: KLH conjugated synthetic peptide derived from human CD16a

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months.

Background: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ding, Peng, et al. "Expression of Tumor-Associated Macrophage in Progression of Human Glioma." Cell Biochemistry and Biophysics (2014): 1-7.Read more>>
  • An G et al. Effects of CCL5 on the biological behavior of breast cancer and the mechanisms of its interaction with tumor‑associated macrophages. Oncol Rep. 2019 Dec;42(6):2499-2511. Read more>>
  • Mu JY et al. Astragalus polysaccharide restores activation of NK cells in radiation therapy of tumors. Int J Clin Exp Med 2019;12(7):8609-8621.Read more>>
VALIDATION IMAGES

Lane 1: Raw264.7 cell lysates probed with CD16 Polyclonal Antibody, Unconjugated (bs-6028R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Rat Thymus cell lysates; Lane 2: Mouse Lymph node cell lysates probed with CD16 Polyclonal Antibody, Unconjugated (bs-6028R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD16 Polyclonal Antibody, Unconjugated (bs-6028R) at 1:400 overnight at 4°C, DAB staining.