bs-60511R
[Primary Antibody]
GSK-3alpha Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: GSK-3alpha
Clonality: Polyclonal
Isotype: IgG2a/Kappa
Swiss Prot: P49840
Purification: Protein G.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications: WB(1:300-5000)
Cross Reactive Species:
Human
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rat IgG H&L (HRP) Lysate: 293, U87MG Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 51 kDa Observed MW: 51 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rat IgG H&L (HRP) Lysate: Rat lung Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 51 kDa Observed MW: 51 kDa