bs-6065R [Primary Antibody]
TCF3 Polyclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TCF3

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6929

Source: KLH conjugated synthetic peptide derived from human TCF3/E2A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-TCF3 Polyclonal Antibody, Unconjugated (bs-6065R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: A549 lysates; Lane 2: Raji lysates probed with TCF3 Polyclonal Antibody, Unconjugated (bs-6065R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Human Jurkat cell lysates; Lane 2: Human K562 cell lysates probed with TCF3 Polyclonal Antibody, Unconjugated (bs-6065R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.