bs-6109R [Primary Antibody]
SNF5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SNF5

Immunogen Range: 301-385/385


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6598

Swiss Prot: Q12824

Source: KLH conjugated synthetic peptide derived from human SNF5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

SNF5 is involved in transcriptional activation. The SWI/SNF complex is required for the induced expression of a large number of genes. This complex alters chromatin structure to facilitate binding of gene-specific dedicated transcription factors. SNF5 also funtions as a tumor suppressor gene and is frequently mutated in malignant rhabdoid tumors. It is also involved in adipocyte differentiation.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Cow
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • McAndrew, Michael J., et al. "Chromatin remodeler recruitment during macrophage differentiation facilitates transcription factor binding to enhancers in mature cells." Journal of Biological Chemistry 291.35 (2016): 18058-18071.Read more>>
VALIDATION IMAGES

Antigen: bs-6109P, 0.2ug/100ul \n\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \n\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\n\nTMB staining;\n\nRead the data in MicroplateReader by 450


Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SNF5 Polyclonal Antibody, Unconjugated (bs-6109R) at 1:200 overnight at 4°C, DAB staining.