DATASHEET
Host:
Rabbit
Target Protein:
EID1
Immunogen Range:
111-187/187
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
23741
Source:
KLH conjugated synthetic peptide derived from human EID1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
EID1 ia an acetyltransferase enzyme. It acetylates histones, giving a specific tag for transcriptional activation. EID1 also acetylates non histone proteins, like NCOA3 coactivator. It Mediates cAMP gene regulation by binding specifically to phosphorylated CREB protein and as coactivator, augments the activity of phosphorylated CREB to activate transcription of cAMP responsive genes.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EID1) Polyclonal Antibody, Unconjugated (bs-6138R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EID1) Polyclonal Antibody, Unconjugated (bs-6138R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.