bs-6180R [Primary Antibody]
Rab3A+Rab3B+Rab3C+Rab3D Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Rab3A+Rab3B+Rab3C+Rab3D

Immunogen Range: 101-150/220


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human Rab3A+Rab3B+Rab3C+Rab3D

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has four known isoforms: 3A, 3B, 3C and 3D. These proteins are expressed in the highest concentration in the brain and endocrine tissues and are involved in regulated exocytosis of hormones and neurotransmitters. Specifically Rab3's are thought to play a role in presynaptic vesicle trafficking and priming.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 24-27


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Rab3A+Rab3B+Rab3C+Rab3D Antibody Polyclonal Antibody, Unconjugated (bs-6180R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.


Mouse eye lysates probed with Rab3A+Rab3B+Rab3C+Rab3D Polyclonal Antibody, Unconjugated (bs-6180R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.