bs-6199R [Primary Antibody]
SHC3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SHC3

Immunogen Range: 501-594/594


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 53358

Swiss Prot: Q92529

Source: KLH conjugated synthetic peptide derived from human SHC3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 64


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti SHC3 Polyclonal Antibody, Unconjugated (bs-6199R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 65kD. Observed band size: 65kD


Lane 1: Kindey lysates probed with SHC3 Polyclonal Antibody, Unconjugated (bs-6199R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.