bs-6221R [Primary Antibody]
Thyroid Hormone Receptor Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: THRA1

Immunogen Range: 81-180/490


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7067

Swiss Prot: P10827

Source: KLH conjugated synthetic peptide derived from human THR

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Sheep
Pig
Horse
Chicken
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun, Yan, et al. "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." Theriogenology (2014).Read more>>
  • Kamakshi Prudhula Devalraju. et al. Reduced thyroxine production in young household contacts of tuberculosis patients increases active tuberculosis disease risk. Jci Insight. 2021 Jul;6(13):148271Read more>>
  • Custodio, Raly James Perez. et al. Low striatal T3 is implicated in inattention and memory impairment in an ADHD mouse?model overexpressing thyroid hormone-responsive protein. Commun Biol. 2021 Sep;4(1):1-14Read more>>
VALIDATION IMAGES

Rat liver lysates probed with Thyroid Hormone Receptor Polyclonal Antibody, Unconjugated (bs-6221R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Thyroid Hormone Polyclonal Antibody, Unconjugated (bs-6221R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Liver cell lysates probed with Thyroid Hormone Receptor Polyclonal Antibody, Unconjugated (bs-6221R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.