Host: Rabbit
Target Protein: Thyroid hormone receptor alpha
Immunogen Range: 81-180/490
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7067
Swiss Prot: P10827
Source: KLH conjugated synthetic peptide derived from human THR
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 55
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Sheep
Pig
Horse
Chicken
Rabbit
GuineaPig
For research use only. Not intended for diagnostic or therapeutic use.
- Sun, Yan, et al. "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." Theriogenology (2014).Read more>>
- Kamakshi Prudhula Devalraju. et al. Reduced thyroxine production in young household contacts of tuberculosis patients increases active tuberculosis disease risk. Jci Insight. 2021 Jul;6(13):148271Read more>>
- Custodio, Raly James Perez. et al. Low striatal T3 is implicated in inattention and memory impairment in an ADHD mouse?model overexpressing thyroid hormone-responsive protein. Commun Biol. 2021 Sep;4(1):1-14Read more>>
Rat liver lysates probed with Thyroid Hormone Receptor Polyclonal Antibody, Unconjugated (bs-6221R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Thyroid Hormone Polyclonal Antibody, Unconjugated (bs-6221R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Liver cell lysates probed with Thyroid Hormone Receptor Polyclonal Antibody, Unconjugated (bs-6221R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.