bs-6253R [Primary Antibody]
CAMKK2 Polyclonal Antibody
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Host: Rabbit

Target Protein: CAMKK2

Immunogen Range: 501-588/588


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10645

Source: KLH conjugated synthetic peptide derived from human CAMKK2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Seven transcript variants encoding six distinct isoforms have been identified for this gene. Additional splice variants have been described but their full-length nature has not been determined. The identified isoforms exhibit a distinct ability to undergo autophosphorylation and to phosphorylate the downstream kinases. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Flow-Cyt(Flow-Cyt=1ug/Test)

Predicted Molecular Weight: 55


Cross Reactive Species: Human
Mouse
Rat
Pig
Sheep
Others

Predicted Cross Reactive Species: Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.Read more>>
  • Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology 313 (2016): 149-158.Read more>>
  • Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
  • Yao C et al. Data Mining and Validation of AMPK Pathway as a Novel Candidate Role Affecting Intramuscular Fat Content in Pigs. Animals (Basel). 2019 Apr 1;9(4). pii: E137. Read more>>
  • Yang WR et al. Oxidative stress mediates heat-induced changes of tight junction proteins in porcine Sertoli cells via inhibiting CaMKKβ-AMPK pathway. Theriogenology. 2019 Sep 19;142:104-113. Read more>>
  • Zhang Q et al. CTRP13 attenuates the expression of LN and CAV-1 Induced by high glucose via CaMKK/AMPK pathway in rLSECs. Aging (Albany NY). 2020 Jun 17;12(12):11485-11499.Read more>>
  • Zuodong Chen. et al. Oxidative stress induced by hydrogen peroxide promotes glycolysis by activating CaMKK/LKB1/AMPK pathway in broiler breast muscle. Poultry Sci. 2021 Dec;:101681Read more>>
  • Yuge Jiang. et al. Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake.. FRONT NUTR. 2022 Nov;9:1039753-1039753Read more>>
  • Xing Guo. et al. Microcystin leucine arginine induces human sperm damage: Involvement of the Ca2+/CaMKK/AMPK pathway. ECOTOX ENVIRON SAFE. 2023 May;256:114845Read more>>
VALIDATION IMAGES

Lane 1: Hela cell lysates probed with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Cerebral cortex cell lysates; Lane 2: Rat Cerebral cortex cell lysates probed with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:400 overnight at 4°C, DAB staining.


Lane 1: Human Jurkat cell lysates; Lane 2: Human MOLT4 cell lysates; Lane 3: Human HepG2 cell lysates probed with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CAMKK2 Polyclonal Antibody, Unconjugated (bs-6253R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.