DATASHEET
Host:
Rabbit
Target Protein:
MCK10 Tyr513
Modification Site:
Tyr513
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
780
Swiss Prot:
Q08345
Source:
KLH conjugated synthetic phosphopeptide derived from human MCK10 around the phosphorylation site of Tyr513
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants.
VALIDATION IMAGES
HepG2 cell lysates probed with MCK10 (Tyr513) Polyclonal Antibody, Unconjugated (bs-6269R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: Siha cell lysates probed with MCK10 (Tyr513) Polyclonal Antibody, Unconjugated (bs-6269R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates probed with MCK10 (Tyr513) Polyclonal Antibody, Unconjugated (bs-6269R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: Hela cell lysates probed with MCK10 (Tyr513) Polyclonal Antibody, Unconjugated (bs-6269R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human K562 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human HeLa cell lysates probed with phospho-MCK10 (Tyr513) Polyclonal Antibody, Unconjugated (bs-6269R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.