bs-6304R [Primary Antibody]
IFNA4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IFNA4

Immunogen Range: 101-189/189


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3441

Swiss Prot: P05014

Source: KLH conjugated synthetic peptide derived from human IFNA4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Predicted to enable cytokine activity and type I interferon receptor binding activity. Predicted to be involved in several processes, including B cell activation; lymphocyte activation involved in immune response; and positive regulation of peptidyl-serine phosphorylation of STAT protein. Predicted to be located in extracellular region. Predicted to be active in extracellular space. [provided by Alliance of Genome Resources, Apr 2022]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 22


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xueer Liu. et al. Lidocaine inhibits influenza a virus replication by up-regulating IFN4 via TBK1-IRF7 and JNK-AP1 signaling pathways. INT IMMUNOPHARMACOL. 2023 Feb;115:109706Read more>>
  • Xiaoxuan Zhao. et al. Demystifying the impact of prenatal tobacco exposure on the placental immune microenvironment: Avoiding the tragedy of mending the fold after death. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 SepRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IFNA4 Polyclonal Antibody, Unconjugated (bs-6304R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining


Mouse Cerebrum lysates probed with IFNA4 Polyclonal Antibody, Unconjugated (bs-6304R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.