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Host: Rabbit
Target Protein: CH25H
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9023
Swiss Prot: O95992
Source: KLH conjugated synthetic peptide derived from human CH25H
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:400-800)
IHC-F(IHC-F=1:400-800)
IF(IF=1:100-500)
Predicted Molecular Weight: 32
Cross Reactive Species:
Human
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Mukherjee, Pallavi, et al. "Transgenic Tomatoes Expressing the 6F Peptide and Ezetimibe Prevent Diet-induced Increases of Interferon-β and Cholesterol 25-hydroxylase in Jejunum." Journal of Lipid Research (2017): jlr-M076554.Read more>>
- Chattopadhyay et al. Treating the Intestine with Oral ApoA-I Mimetic Tg6F Reduces Tumor Burden in Mouse Models of Metastatic Lung Cancer. (2018) Sci.Rep. 8:9032Read more>>
- Samantha A. Hutchinson. et al. Liver x receptor alpha drives chemoresistance in response to side-chain hydroxycholesterols in triple negative breast cancer. Oncogene. 2021 Mar;:1-12Read more>>
- Ceglia, Simona. et al. An epithelial cell-derived metabolite tunes immunoglobulin A secretion by gut-resident plasma cells. NAT IMMUNOL. 2023 Jan;:1-14Read more>>
- Tran Thanh-Tam. et al. Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss. EXP MOL MED. 2023 Dec;:1-11Read more>>
Lane 1: Human U-87 MG cell Lysates. Probed with CH25H polyclonal Antibody, unconjugated (bs-6480R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CH25H Polyclonal Antibody, Unconjugated (bs-6480R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.